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KMID : 0614619940260010151
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Korean Journal of Gastroenterology
1994 Volume.26 No. 1 p.151 ~ p.156
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Effect of Cholecystokinin on the Formation of Pigment Gallstone by High Carbohydrate, Low Fat and Low Protein Diet in Guinea Pig
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Abstract
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We reported an animal model of pigment gallstone induced by carbohydrate-rich diet mainly composed with rice. However, the machanisms have not been clearly understood.
Under the hypothesis that high-carbohydrate diet(CHO) might induce gallstone formation via the mechanism of the relative bile stasis caused by low secretion of cholecystokin(CCK), the aim of this study was to examine whether exogenous CCK
administration
inhibits gallstone formation induced by CHO in guinea pig.
Male guinea pigs were divided into 3 groups(Group 1 : control chow-fed group, Group 2 : high carbohydrate fed group, Group 3 : high cabohydrate fed and CCK injected group). High carbohydrate diet was 63.2% carbohydrate(45.8% in control chow),
mainly
composed of rice, and Group 3 received a daily injection of cholecystokinin(0.5nmol/kg). After 6 weeks of feeding, the guinea pigs were sacrificed. The stones were analyzed by infrared spectrophotometry and gallbladder bile was analyzed by using
commercial kits.
In group 1, gallstone was found in one case out of 10 animals, in group 2, 9 out of 14(p<0.05 vs Group 1) and in Group 3, 3 out of 12(p<0.05 vs Group 2). There were no differences in the concentrations of total calcium, total bilirubin,
cholesterol,
phospholipid and bile acid of gallbladder bile among 3 groups. The stones were mainly composed of calcium bilirubinate, cholesterol, calcium phosphate, and calcium palmitate, which were similar to human calcium bilirubinate stone. The prevalence
of
stone formation was lowered with administration of CCK in CHO fed animals. A possible mechanism is that exogenous CCK may recover the low CCK release by CHO. It is suggested that bile stasis caused by poor CCK release which may be due to CHO be
the
one
of the contributing factors in the formation of pigment stones in guinea pigs. (Korean J Gastroenterol 1994 ; 26 : 151-156)
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